Expression and Significance of ALDH1 in Cancer Stem Cell-like Characteristics in Breast Cancer Cell Line MCF-7 / 医学研究杂志

ABSTRACT

Objective To investigate the expression and significance of ALDH1 in cancer stem cell-like characteristics in breast cancer cell line MCF-7.Methods RNA lenti-virus particles targeting ALDH1 gene were transfected into MCF-7 cells.Subclones with stable ALDH1 expression were selected by puromycin and named MCF-ALDH1 cells.The mRNA and protein expression of ALDH1 were detected by real-time quantitative PCR and Western blot tests.Cell differentiation,stem cell potential were observed by clone formation assay and suspension sphere-forming assay.The abilities of tumor-formation of cells were detected through inoculating the cells into the nod mouse.To detect the drug-resistance ability of cells by MTT test.Results Compared to the MCF-7 cells,the expression of mRNA and protein of ALDH1 by MCF-ALDH1 cells were significantly increased.Clony forming numbers(128 ±22) of the MCF-AL-DH1 cells was higher than controls(46 ± 15)(P ＜ 0.05),The proportion of sphere-initiating cells in MCF-ALDH1 and controls was (16 ± 2) versus(8.0 ± 1.5,P ＜ 0.05).The average tumor volume of MCF-ALDH1 was greater than controls(P ＜ 0.05).The drug resistance index of MCF-ALDH1 was higher than controls.Conclusion Experimental findings from this study showed that MCF-7 cells highly expressing ALDH1 genes,with higher proliferation and differentiation,in vivo tumorigenicity and potential of stem cells.ALDH1 may be used as an target to cure breast cancer.