Changes of BMP signaling pathway in mammary glands of mice with mammary hyperplasia / 中国病理生理杂志

ABSTRACT

AIM:To determine the expression patterns of bone morphogenetic protein(BMP)signaling com-ponents in the mammary glands with hyperplasia(MGH)and the activation of this signaling pathway.METHODS: The female C57BL/C mice(8-weeks-old)were randomly divided into control group and hormone group with 15 mice in each group.The mice in control group were treated with PBS,while the mice in hormone group were intragastric administration with estradiol valerate(2.5 mg/kg)for 25 d,followed by progesterone(4 mg/kg)peritoneal injection for 5 d.At the end of treatments,the mammary glands were collected to determine the morphological changes, the mRNA expression of BMP signaling components and the phosphorylation level of Smad 1/5/9.RESULTS:In MGH,a part of BMP signaling pathway molecules were differentially expressed(P<0.05),including BMP ligands BMP2,4,5,6,7,9,13 and 14, BMP re-ceptor BMPR1A, and antagonists Chrdl1 and Twsg, whereas the expression levels of some molecules, such as BMP3, BMP12,BMPR1B,BMPR2,Chrd,Chrdl2 and Noggin, were not altered.The phosphorylation levels of Smad 1/5/9 was up-regulated in MGH(P<0.05).CONCLUSION: BMP signaling pathway is activated in MGH through the abnormal expression of its components.BMP signaling pathway may be a new target for MGH therapy.