An analysis of serum biochemical indexes of rats chronically exposed to fluoride / 中华地方病学杂志

ABSTRACT

Objective To study the changes of serum biochemical parameters of rats after subchronic fluoride exposure. Methods Totally 60 Wistar rats were randomly divided into three groups by weight, 20 in each group. Control group drinking distilled water, fluorine groups drinking distilled water containing 60 and 120 mg/L NaF,respectively. After exposed to fluoride for 4 and 12 weeks (n = 10), rats were sacrificed by anesthesia and their serum samples were collected. The biochemical techniques were used to test serum triglyceride (TG), total cholesterol (T-CHO), high density lipoprotein (HDL), low-density lipoprotein cholesterol (LDL), magnesium (Mg2+), alkaline phosphatase (AKP), potassium (K+), phosphate (Pi), and calcium (Ca2+) contents. Results After comparison of serum TG, T-CHO, LDL, Mg2+, K+, Pi, Ca2+levels of rats in the 3 groups at 4 and 12 weeks of fluoride exposure, no statistical significant difference was found (P > 0.05). At 4 and 12 weeks, the HDL levels in fluoride-exposed rats decreased compared to that of the control rats (mmol/L,4 weeks0.40 ± 0.07,0.22 ± 0.05, 0.16 ± 0.04; 12 weeks 0.38 ± 0.32, 0.31 ± 0.14, 0.26 ± 0.13), the 4 week of 60, 120 mg/L fluoride groups were significantly lower than the control group (P < 0.05). After exposed to fluoride for 4 and 12 weeks, the serum concentrations of AKP in the three groups were significantly different statistically(King unit/100 ml,4 weeks16.18 ± 5.03,7.57 ± 5.83,6.01 ± 1.65;12 weeks8.65 ± 3.71,13.70 ± 9.31,18.57 ± 9.16;F=12.38,4.31,P<0.05).At 4 weeks, fluorine group with 60 and 120 mg/L were significantly lower than that in the control group (P < 0.05). However, AKP levels increased with increased fluoride concentrations in rats exposed to fluoride for 12 weeks. Conclusion Subchronic fluoride exposure could change the serum levels of HDL and AKP in rats.