Simultaneous determination of three drugs in saliva by UPLC-MS/MS / 中国法医学杂志
Chinese Journal of Forensic Medicine
; (6): 65-67, 2018.
Article
en Zh
| WPRIM
| ID: wpr-701486
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WPRO
ABSTRACT
Objective A method of determination of methamphetamine, ketamine and morphine in saliva was established by super high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS). Methods The saliva samples after precipitation protein, in the ACQUITY UPLC BEH Phenyl(100mm×2.1mm,1.7μm) column chromatography, after the acetonitrile: 0.3% Formic acid solution gradient elution and through the ESI+ ionization mode MRM analysis and inspection. Results The linearity was estadlished in saliva between 4μg/L and 200μg/L, LOD was 0.2μg/L, LOQ was 4μg/L. the recovery was in the range of 87%-128%. Conclusion The method is simple, rapid and accurate for determination of methamphetamine, ketamine and morphine in saliva.
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WPRIM
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Zh
Revista:
Chinese Journal of Forensic Medicine
Año:
2018
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Article