Effects of Di ao Xin xue Kang on connexin 43 of myocardial gap junction after acute ischemia injury / 中国基层医药

ABSTRACT

Objective To observe the effects of Di ao Xin xue Kang on connexin 43 ( Cx43 ) , in order to make clear the protective mechanism against ischemia -induced arrhythmias .Methods The rats were randomly divided into 3 groups according to the digital table:SM operation group(group SM,n=10),ischemia group(group IM,n=10),Di ao Xin xue Kang group(group DA,n=10).Myocardial infarction was induced by legation the left anterior descending coronary artery for 120 min.The expression of Cx43 of the ischemic myocardium was studied by immunohistochemistry technique .The ventricular arrhythmia was observed , and MDA, SOD levels were measured . Results Compared with the SM group ,the arrhythmia score[(3.8 ±0.7)points vs.(0.7 ±0.2)points,t=13.466, P<0.01]and serum MDA level[(8.3 ±0.8)nmol/mL vs.(4.2 ±0.7)nmol/mL,t=12.197,P<0.01]in the IM group were significantly increased,SOD activity in the IM group[(68.2 ±5.5)U/mL vs.(144.1 ±7.9)U/mL,t=24.932,P<0.01]decreased,and serum Cx43 content[(0.39 ±0.06) vs.(0.92 ±0.05),t=21.459,P<0.01] significantly decreased.Compared with IM group,the arrhythmia score of the DA group [(2.6 ±0.8) vs.(3.8 ± 0.7),t=3.570,P<0.01]decreased,and serum Cx43 content[(0.60 ±0.08) vs.(0.39 ±0.06),t=6.641,P<0.01]increased and distributed regularly.Conclusion Di ao Xin xue Kang can effectively alleviate Cx43 degrading induced by acute myocardial infarction ,the mechanism of this effect is associated with resisting oxidative stress .