Preparation and in vitro ultrasound/photoacoustic imaging of folate receptor-targeted, doxorubicin and melanin-loaded multifunctional contrast media / 中国介入影像与治疗学

ABSTRACT

Objective To prepare the folate receptor-targeted,doxorubicin and melanin-loaded multifunctional contrast media,and to observe its characteristics,targeting,imaging performance and cell toxicity on human MDA-MB-231 cell lines in vitro.Methods The folate receptor-targeted,doxorubicin and melanin-loaded multifunctional contrast media wasprepared using single emulsion method and freeze-drying and C3 Fs-inflation.Its size and drug loading efficiency were measured with Malvern instrument and UV-visible spectrophotometry.Its enhanced respectively ultrasound and photoacoustic imaging were observed in vitro model.The targeting performance of contrast media and its cell toxicity werechecked after co-incubated with human MDA-MB-231 cell lines.Results The average diameter of contrast media was (659.60±27.56)nm,Zeta potential was (-38.90±4.00)mV,and the drug loading capacity was 85.72 μg/mg.In vitro,the targeting experiment showed that a large number of folate receptor-targeted contrast media were aggregated and adhered firmly to the MDA-MB-231 cells.The folate receptor-targeted contrast media significantly inhibited the proliferation of human MDA-MB-231 cells.The ultrasound and photoacoustic imaging of folate receptor-targeted contrast media were significantly enhanced.Conclusion The folate receptor-targeted,doxorubicin and melanin-loaded multifunctional contrast media is a set of targeting treatment,enhancing ultrasound and photoacoustic imaging,having potential of perfect molecular probe for targeting imaging and treatment of human breast cancer.