Expression and significance of IL-35 in chronic sinusitis with nasal polyps / 中国免疫学杂志

ABSTRACT

Objective:To observe the expression of IL-35 in chronic sinusitis with nasal polyps(CRSwNP),and to complement the pathogenesis.Methods:The clinical data and nasal polyp tissues of CRSwNP who were hospitalized from May 2016 to April 2017 were randomly selected.The subjective symptoms were scored by visual analogue scale (VAS),the nasal endoscopy score by Lanza-Kennedy,and the CT score by Lund-Mackay.The IL-35 mRNA were detected by Real-time PCR in nasal polyp tissues,while the IL-35, IL-17 and IL-10 were detected by ELISA.Results: A total of 51 patients of the clinical data and nasal polyp tissues were collected.Among them,30 males and 21 females had the mean age of (43.3 ± 15.1) years,the VAS score was (5.5 ± 1.7),the Endoscopy score was(6.4±2.0),the CT score was(11.7±3.8).Our results of Real-time PCR and ELISA analysis showed that IL-35 mRNA and protein expression was significantly decreased in CRSwNP compared with normal nasal mucosa (P<0.05).But IL-17 and IL-10 were significantly increased by ELISA in CRSwNP compared with normal nasal mucosa (P<0.05).Conclusion: These data suggest that IL-35 may play an important role in the development and progression of CRSwNP.