Evaluation of therapeutic effects of transplantation of human stem cells from apical papilla ameliorates on collagen-induced arthritis / 中国免疫学杂志

ABSTRACT

Objective:To study the effect of transplantation of stem cells from the apical papilla (SCAP) to collagen-induced arthritis (CIA).Methods:Twenty DBA/1J mice with collagen Ⅱ-induced arthritis were equally divided into 2 groups and treated with human SCAP and PBS,respectively,with other 6 normal mice as negative control.Inflammation was evaluated by measuring clinical score,TNF-α and anti-CⅡ antibody levels (ELISA),histological analyses and micro-CT analyses.The levels of CD4+Th cells subsets in spleens were assessed by flow cytometric analysis.Results:System infusion of SCAP could significantly reduce the severity of CIA, and resume the balance of Th cells subsets.Conclusion:SCAP transplantation can induce immune tolerance and ameliorate the CIA in-flammation.