Regulation of the apoptosis on the rat granulosa cell: effects of EGF, TGF-alpha / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 2439-2446, 2004.
Artículo
en Coreano
| WPRIM
| ID: wpr-70287
ABSTRACT
OBJECTIVE:
To evaluate the effect of the epidermal growth factor (EGF) and the transforming growth factor-alpha (TGF-alpha) on the apoptosis of the rat granulosa cells.METHODS:
For this study, granulosa cells were isolated from female rats, and granulosa cells were cultured in the presence or absence of different autocrine/paracrine factors such as EGF and TGF-alpha. In order to investigate the apoptotic process, the number of apoptotic cells were counted using Annexin V staining via flow cytometry. In addition, the expression levels of the Bcl-2, ICE and BAX genes were checked.RESULTS:
c-AMP induced apoptosis was inhibited neither by EGF nor by TGF-alpha, but FSH induced apoptosis was inhibited by EGF and TGF-alpha. Both EGF and TGF-alpha decreased the rate of apoptosis in the serum free cultured media in a time dependent manner by inducing survival gene expression such as Bcl-2 and suppressing death gene expression such as ICE and BAX.CONCLUSION:
EGF and TGF-alpha can be used to decrease the apoptotic rate of the rat granulosa cells. It is hoped to conduct a similar study on the function of these growth factors in the human granulosa cells, and to use EGF and TGF-alpha for reducing the apoptosis rate of the human granulosa cells in IVF.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Factores de Crecimiento Transformadores
/
Expresión Génica
/
Factor de Crecimiento Transformador alfa
/
Apoptosis
/
Anexina A5
/
Péptidos y Proteínas de Señalización Intercelular
/
Factor de Crecimiento Epidérmico
/
Esperanza
/
Citometría de Flujo
/
Células de la Granulosa
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Año:
2004
Tipo del documento:
Artículo
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