Expression and purification of human placental growth factor 2 using the Pichia pastoris expression system / 中国药科大学学报
Journal of China Pharmaceutical University
;
(6): 376-380, 2018.
Artículo
en Chino
| WPRIM
| ID: wpr-704349
ABSTRACT
To prepare the human placental growth factor 2 (PIGF-2 ),human PIGF-2 gene was cloned into pPIC9K vector to construct the recombinant pPIC9K-PIGF-2 vector. Linearized recombinant pPIC9K-PIGF-2 was transformed into Pichia pastoris by electroporation. YPD-Geneticin plate was used to screen geneticin hyper-resistant colonies. The positive colonies were verified by PCR. Results of SDS-PAGE and Western blot showed that recom-binant human PIGF-2 was expressed after being induced by methanol. Using its characteristic heparin binding, recombinant human PIGF-2 was successfully purified by heparin affinity column chromatography.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of China Pharmaceutical University
Año:
2018
Tipo del documento:
Artículo
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