Comparative Study of the Effect of High Intensity Interval Training and Moderate Intensity Continuous Training on Metabolic Phenotype in C57BL/6 Mice / 中国运动医学杂志

ABSTRACT

Objective To examine the effect of high intensity interval training(HIIT) and moderate intensity continuous training(MICT) on the body component,serum globular adiponectin and skeletal muscle autophagy in C57BL/6 mice.Methods Thirty-six four-week-old C57BL/6 mice were randomly assigned to the control group,MICT group and HIIT groups.The MICT group underwent 60 minutes' aerobic continuous treadmill training at 12 m/min(75％ VO2max) once a day,5 times/week for 6 weeks,while the HIIT group exercised for 1min at 20 m/min(85％ VO2max) followed by 1 min at 8 m/min (50％ VO2max) for alternating 12 cycles.The body weight,body mass index(BMI),total body water,fat mass and citrate synthase(CS) of the quadriceps femoris were measured using ImpediVET experimental animal body composition analyzer.Mice were sacrificed after the analysis,and the level of serum gAcrp30 was detected using the enzyme linked immunosorbent assay.Western blotting was used to detect the expression of pAMPK-Thr172,LC3II/I,Beclin1 and p62 protein in skeletal muscle tissues.Results After 6-week exercise,the body weight of MICT and HIIT mice decreased significantly compared with the sedentary control group.BMI and fat mass of the HIIT group were significantly lower than the control group,but there were no significant differences between the MICT and control groups in the above two measurements.The activity of CS and serum gAcrp30 in HIIT group was significantly higher than the control group.Moreover,the expression of pAMPK-Thr172,LC3II/I and Beclin1 increased significantly in the skeletal muscle after HIIT intervention while the expression of p62 decreased compared with the control group.Conclusion Compared with the MICT,HIIT has more significant effects to reduce body fat and BMI,and activate the autophagy of skeletal muscles.