Simultaneous Determination of Liquiritin and Glycyrrhizic Acid in Fuzi Lizhong Pills (Condensed Pills) by HPLC / 中国药房
China Pharmacy
;
(12): 5147-5149, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-704496
ABSTRACT
OBJECTIVE:
To establish the method for simultaneous determination of liquiritin and glycyrrhizic acid in Fuzi lizhong pills (condensed pills).METHODS:
HPLC method was adopted.The determination was performed on WondaSil C18 column with mobile phase consisted of acetonitfile-0.05% phosphate solution (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 237 nm,and column temperature was 35 ℃.The sample size was 10 μL.RESULTS:
The linear range ofliquiritin and glycyrrhizic acid were 9.68-96.8 μg/mL(r=0.999 1)and 14.08-140.8 μg/mL(r=0.999 2).The limits of quantitation were 0.2,0.3 μg/mL,and the limits of detection were 0.1,0.01 μg/mL.RSDs of precision,stability and reproducibility tests were all lower than 2.0%.The average recoveries were 98.9%-101.2% (RSD=0.62%,n=9),98.6%-101.5% (RSD=1.06%,n=9).CONCLUSIONS:
The method is simple and accurate,and can be used for simultaneous determination of liquiritin and glycyrrhizic acid in Fuzi lizhong pills (condensed pills).
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
2017
Tipo del documento:
Artículo
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