Determination of Related Substance in Posaconazole Raw Material by HPLC / 中国药房
China Pharmacy
;
(12): 5157-5159, 2017.
Artículo
en Chino
| WPRIM
| ID: wpr-704499
ABSTRACT
OBJECTIVE:
To establish a method for the determination of related substance in posaconazole raw material.METHODS:
HPLC method was adopted.The separation was performed on Zorbax Eclipse Plus C18 column with mobile phase consisted of 0.05 mol/L ammonium acetate solution (pH adjusted to 4.0 using acetic acid)-acetonitrile (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 262 ran,and the column temperature was 30 ℃.The sample size was 20 μL.RESULTS:
The linear range of intermediate I was 0.05-5.0 μg/mL (r=0.999 9);the limit of quantitation was 0.05 μg/mL,the limit of detection was 0.013 μg/mL;RSDs of precision test,stability test and repeatability test were lower than 2.0%;the recoveries was 98.00%-101.69% (RSD=1.22%,n=9).CONCLUSIONS:
The method is simple,accurate and suitable for the determination of related substance in posaconazole raw material.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Pharmacy
Año:
2017
Tipo del documento:
Artículo
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