Study on proliferation and apoptosis of paclitaxel to liver carcinoma cell line HepG2 and hepatocytes in vitro / 中国药理学通报

ABSTRACT

Aim To explore the influence of paclitaxel in the proliferation and apoptosis of liver carcinoma cell line HepG2 and hepatocytes in vitro. Methods HepG2 cells and hepatocytes were divided into negative control group and different concentrations(5, 10, 20, 40, 80 μg·L-1) paclitaxel groups. The inhibitory rates of proliferation of HepG2 cells and hepatocytes treated by different concentrations of paclitaxel for 24, 48 and 72 hours were assessed by MTT method. The apoptotic rates and cell cycle of HepG2 cells and hepa-tocytes treated by different concentrations of paclitaxel were measured by flow cytometry after Annexin V/PI staining. Results MTT results showed that paclitaxel inhibited the proliferation of HepG2 cells and hepato-cytes in a concentration-dependent manner ( P <0.05) . Paclitaxel induced morphological changes of apoptosis in the HepG2 cells and hepatocytes by using Hoechst 3328 staining. The flow cytometry results showed the cell cycle changes of HepG2 cells and hep-atocytes treated by different concentrations of paclitaxel for 24 h. Compared with negative control group, the percentages of HepG2 cells and hepatocytes in G2/M phase increased ( P < 0.05 ) . The G2/M block in-creased with the drug concentration. With the increase in Paclitaxel, G2/M phase significantly increased( P<0.05), G0/G1phase decreased(P <0.05), but no significant change occurred in S phase ( P >0.05 ) . Meanwhile, paclitaxel induced apoptosis in HepG2 cells and hepatocytes. However, the apoptotic rate of HepG2 cells induced by paclitaxel was significantly higher than that of hepatocytes, and the apoptotic rate of HepG2 cells increased gradually with the increase of paclitaxel concentration. Conclusion Paclitaxel can inhibit the proliferation of HepG2 cells and hepato-cytes, which may be achieved by inducing apoptosis and cell cycle arrest in G2/M phase.