Cardioprotective effect of Danshen injectable powder on pressure overload-induced ventricular remodeling in rats and isoproterenol induced myocardial injury in mice / 中国药理学与毒理学杂志

ABSTRACT

OBJECTIVE To determine the effects of Danshen injectable powder on myocardial remodeling in pressure overload rats and isoproterenol(ISO)injection mice.METHODS SD Rats were subject to abdominal aortic constriction(AAC)surgery to develop pressure overload.Therapeutic effect of Danshen injectable powder was assessed six weeks after AAC surgery. The lactic dehydrogenase (LDH) and creatine kinase (CK) levels in serum, biometric, echocardiographic parameters, interstitial fibrosis, the expression of DJ-1 and SOD2 were then measured. The myocardial impairment in mice was induced by ISO injection. LDH and CK in serum, biometric, interstitial fibrosis were measured to investigate the cardioprotective effect of Danshen injectable powder. Matrix-assisted laser desorption mass spectrometry imaging(MALDI-MSI)was used to detect the changes of 60-1000 Da molecules in mice heart tissue. RESULTS In AAC rats, we observed compensatory hypertrophy and perivascular fibrosis,however cardiac function had not progressed to deterioration.The levels of LDH and CK were decreased significantly in the Danshen injectable powder groups(P<0.05,P<0.05).Danshen injectable powder could alleviate the perivascular collagen deposits but not cardiac hypertrophy in AAC rats. Furthermore,Danshen injectable powder enhanced the expression levels of SOD2 and DJ-1 in cardio-myocytes (P<0.05, P<0.01). In ISO injection mice, the levels of HWI and LDH were decreased signifi-cantly in drug treatment group compared with model group (P<0.01, P<0.01). In the MALDI-MSI of mice heart section,Danshen injectable powder can improve the reduction of energy metabolism-related substances including adenosine, creatine and ADP induced by ISO impairment. CONCLUSION Danshen injectable powder can attenuate perivascular fibrosis and improve the expression of anti-oxidative stress-related proteins SOD2,DJ-1 in the rats with pressure overload-induced left ventricular remodeling.Danshen injectable powder has cardioprotective effect on ISO impairment in mice which is probably related to improvement of energy metabolism.