1H-NMR-based Metabolomic approach to evaluating total flavonoids of Ocimum Basilicum Linn in apolipomtein E knockout mice / 中国药理学与毒理学杂志

ABSTRACT

OBJECTIVE To observe the effects of serum metabolites by using 1H-NMR-based metabonomic approach to explore the possible mechanisms of total flavonoids in Ocimum Basilicum Linn (OBL) on atherosclerosis in apolipomtein E knockout (ApoE-/-) mice. METHODS Six-week-old male apoE knockout mice were divided into four groups(n=10)and fed with high fet dietmodel,Simv-astatin, OBL-H, OBL-M and OBL-L groups. The homogeneous male mice of C57BL/6J were used as the normol group and fed with normal chow diet. After 14 weeks,1H-NMR technology was used to ex-plore the variability of serum metabolites by the method of PLS-DA and OPLS-DA. RESULTS Com-pared with normal group,Model group showed a significant increase in the serum levels of VLDL,LDL, β-hydroxyisobutyrate,lactate,myo-inositol and showed a significant decrease in the serum levels of al-anine, glutamine, proline, carnitine, methylamine, citrate, creatine, choline, taurine, pyruvate, β-glu-cose, α-glucose, glycine, lysine. Combined with model group OBL-H, OBL-M, OBL-L groups showed the effects of regulating the levels of different metabolites of the glucose,lipid and amino acid metabo-lism.CONCLUSION The anti-atheros-clerotic activity of total flavonoids in Ocimum Basilicum Linn may be related not only to regulation of lipid metabolism,but also glycometabolism and amino acid metabolism.