Determination of Potassium Sorbate in Xiao'er Kechuanling Oral Solution by HPLC / 中国药师

ABSTRACT

Objective:To develop a method for the determination of potassium sorbate in Xiao 'er Kechuanling oral solution by HPLC.Methods:A Phenomenex Gemini C18 column (250 mm ×4.6 mm, 5 μm) was used, and the mobile phase was 0.02 mol· L-1 sodium dihydrogen phosphate (adjusting pH to 4.0 with phosphoric acid)-acetonitrile (8020).The flow rate was 1.0 ml· min-1 and the detection wavelength was set at 262 nm.The column temperature was 30℃and the injection volume was 20μl.Results:The linear range of potassium sorbate was 8.584-85.840μg· ml-1(r=0.9999).The recovery of potassium sorbate was 100.0％and the RSD was 0.47％(n=9).The limit of detection was 0.43 ng and the limit of quantitation was 1.29 ng.Conclusion:After the valida-tion, the method can be used for the assay of potassium sorbate in Xiao'er Kechuanling oral solution .