Effects of Two Drugs on the Respiration of Zebrafish / 中国药师

ABSTRACT

Objective:To investigate the effects of two drugs on the asphyxia oxygen concentration and the oxygen consumption rate of zebrafish,and explore the possibility of zebrafish as a drug model and the feasibility of oxygen microelectrode used for drug screening. Methods:The zebrafish were treated with the drugs for 5 days,and transferred into the asphyxia chambers. The solute oxy-gen concentration was assessed continuously until the asphyxia condition. The asphyxia oxygen concentration,asphyxia time and mass specific oxygen consumption rate were determined. Results:The results showed that the asphyxia time,asphyxia concentration and ox-ygen consumption rate of the zebrafish were not significantly changed by deproteinized calfblood extractives injection(P>0.05),sug-gesting no reduction in hypoxia tolerance ability. The asphyxia time and oxygen consumption rate of the zebrafish were not significantly changed,either (P>0.05),however,the asphyxia concentration was increased significantly by cervus and cucumis polypeptide injec-tion (P<0.05),suggesting the reduction in hypoxia tolerance ability. Conclusion:It is suggested that the hypoxia tolerance ability of zebrafish can be significantly decreased by the solution of cervus and cucumis polypeptide. The results also suggest that zebrafish can be used as an experimental animal in medicine studies,and the oxygen microelectrode method can be used in drug screening.