Effects of Jianpi Qingchang Decoction Combined with Calcitriol on IL-17 and IFN-γ in Ulcerative Colitis Rats / 中国中医药信息杂志

ABSTRACT

Objective To investigate the effects of Jianpi Qingchang Decoction combined with Calcitriol on the expressions of IL-17 and IFN-γ in colitis in rats with ulcerative colitis; To discuss the mechanism of action. Methods Experimental rats were randomly divided into control group, model group, Mesalazine group, Calcitriol group, Jianpi Qingchang Decoction+Calcitriol group, with 10 rats in each group. Except for the control group, other groups were given TNBs for modeling. The administration began on the 3rd day after modeling. All rats were weighed and sacrificed on the 11th day after administration. The disease activity index (DAI) were calculated and the colon histopathological (HPS) were evaluated under microscope. The mRNA and protein expressions of IFN-γ and IL-17 in colonic mucosa were detected by RT-PCR and immunohistochemistry. Results Compared with the control group, the DAI and HPS of the model group increased significantly (P<0.01); Compared with the model group, the DAI and HPS of administration groups were reduced significantly (P<0.05, P<0.01), and the mRNA and protein expressions of IFN-γ and IL-17 in Mesalazine group and Jianpi Qingchang Decoction+Calcitriol group decreased significantly (P<0.01). Conclusion Jianpi Qingchang Decoction combined with Calcitriol can down-regulate IL-17 expression, decrease the activity of immune cells, reduce the secretion of inflammatory cells, and lessen the damage of colonic mucosa by regulating Th17 cells.