Effects of Dijincao Tablets on T-lymphocytes subsets and cytokines in patients with immune thrombocytopenia / 中成药

ABSTRACT

AIM To observe the effects of Dijincao Tablets (Euphorbia humifusa Willd.) on T-lymphocytes subsets and cytokines in patients with immune thrombocytopenia (ITP).METHODS Fifty-six patients who were first diagnosed ITP at hematology department of our hospital from Jan.2014 to Dec.2015 were divided into observation group (twenty-eight cases) and control group (twenty-eight cases) by Stratified blocked randomization.The patients in the observation group were given Dijincao Tablets (4.8 g,tid) based on the conventional treatment,while the patients in the control group only accepted conventional treatment according to guidelines.Adverse events were collected during the treatment.Fasting venous blood was obtained from all patients in the morning on the second day of admission and the weekend of the forth week (30th day after admission) to detect observation indexes,and then the comprehensive effects were evaluated.The levels of T-lymphocytes subsets of CD3 +,CD4 +,CD8 + and CD4 +/CD8 + were examined by flow cytometry,and serum cytokine levels of IL-2,IFN-γ,IL-4 and IL-10 were determined by ELISA.RESULTS The total effective rate was 92.9％ in the observation group,which was higher than 82.1％ in the control group.The platelet level in the observation group was significantly higher than that in the control group.Compared with the control group,the levels of CD3 +,CD4 + and CD4 +/CD8 + in the observation group were significantly increased,the levels of IL-2 and IFN-γwere significantly decreased,and the ratio of IFN-γ/IL-4 was significantly decreased.CONCLUSION Dijincao Tablets can correct and regulate the disorder and ratio of T-lymphocyte subsets,and decrease the ratio of IFN-γ/IL-4,then achieve therapeutic effect on patients with immune thrombocytopenia.