Establishment of HPLC fingerprints of Geranium strictipes and pattern recognition / 中成药

ABSTRACT

AIM To establish HPLC fingerprints of Geranium strictipes R.Kunthand to perform pattern recognition.METHODS The analysis of 70％ methanol extract of G.strictipes was performed on a 25℃ thermostatic ZORBAX SB-C18 column(250 mm× 4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-water in a gradient elution manner,and the detection wavelength was set at 254 nm.The results were analyzed by cluster analysis and principal component analysis.RESULTS There were thirteen common peaks in the HPLC fingerprints of twenty-one batches of samples with the similarities of more than 0.894.Twenty-one batches of samples were divided into two categories.The cumulative contribution rate of the first principal component was 31.067％.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of G.strictipes.