Effects of oxidized low density lipoprotein on the function of dendritic cells in mice / 中华微生物学和免疫学杂志

ABSTRACT

Objective To investigate the effects of oxidized low density lipoprotein (OxLDL) on dendritic cells (DC) in order to provide an experimental basis for better understanding the role of DC in ath-erosclerosis. Methods Mouse splenic DC, arterial DC and na?ve T cells were sorted by immunomagnetic sorting and fluorescence-activated cell sorting. Expression of Chop,Caspase-3,heme oxygenase 1 (HMOX-1),Toll-like receptors (TLR), CD36 and CD86 at mRNA level in DC was detected by quantitative real-time PCR. To analyze the influence of DCs on T cell differentiation,IFN-γ and IL-17 that secreted by anti-gen-specific na?ve CD4+T cells which were co-cultured with DC were detected by flow cytometry. PEMS3.1 software was used for statistical analysis.Differences between groups were compared using one-way ANOVA. Results OxLDL promoted the expression of Chop, Caspase-3 and HMOX-1 in mouse splenic and arterial wall DC at mRNA level,which was inhibited by N-Acetylcysteine (NAC), an inhibitor of reactive oxygen species (ROS). OxLDL induced an increased expression of CD36,CD86 and TLR4 at mRNA level on arte-rial wall DC. OxLDL promoted the DC-induced differentiation of na?ve CD4+CD62L+T cells into Th17 cells. Conclusion OxLDL promotes the expression of Chop,Caspase-3 and HMOX-1 at mRNA level in DC in a ROS-dependent manner and enhances the expression of CD36,TLR4 and CD86. Moreover,it promotes the DC-induced differentiation of na?ve CD4+T cells into Th17 cells.