Comparison of Four Human Papillomavirus Genotyping Methods: Next-generation Sequencing, INNO-LiPA, Electrochemical DNA Chip, and Nested-PCR
Annals of Laboratory Medicine
;
: 139-146, 2018.
Artículo
en Inglés
| WPRIM
| ID: wpr-713685
ABSTRACT
BACKGROUND:
Human papillomavirus (HPV) infection causes cervical cancer, thus necessitating early detection by screening. Rapid and accurate HPV genotyping is crucial both for the assessment of patients with HPV infection and for surveillance studies.METHODS:
Fifty-eight cervicovaginal samples were tested for HPV genotypes using four methods in parallel nested-PCR followed by conventional sequencing, INNO-LiPA, electrochemical DNA chip, and next-generation sequencing (NGS).RESULTS:
Seven HPV genotypes (16, 18, 31, 33, 45, 56, and 58) were identified by all four methods. Nineteen HPV genotypes were detected by NGS, but not by nested-PCR, INNO-LiPA, or electrochemical DNA chip.CONCLUSIONS:
Although NGS is relatively expensive and complex, it may serve as a sensitive HPV genotyping method. Because of its highly sensitive detection of multiple HPV genotypes, NGS may serve as an alternative for diagnostic HPV genotyping in certain situations.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ADN
/
Neoplasias del Cuello Uterino
/
Tamizaje Masivo
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Genotipo
/
Métodos
Tipo de estudio:
Estudio de tamizaje
Límite:
Humanos
Idioma:
Inglés
Revista:
Annals of Laboratory Medicine
Año:
2018
Tipo del documento:
Artículo
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