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Designing Tyrosinase siRNAs by Multiple Prediction Algorithms and Evaluation of Their Anti-Melanogenic Effects
Biomolecules & Therapeutics ; : 282-289, 2018.
Artículo en Inglés | WPRIM | ID: wpr-714738
ABSTRACT
Melanin is a pigment produced from tyrosine in melanocytes. Although melanin has a protective role against UVB radiation-induced damage, it is also associated with the development of melanoma and darker skin tone. Tyrosinase is a key enzyme in melanin synthesis, which regulates the rate-limiting step during conversion of tyrosine into DOPA and dopaquinone. To develop effective RNA interference therapeutics, we designed a melanin siRNA pool by applying multiple prediction programs to reduce human tyrosinase levels. First, 272 siRNAs passed the target accessibility evaluation using the RNAxs program. Then we selected 34 siRNA sequences with ΔG ≥−34.6 kcal/mol, i-Score value ≥65, and siRNA scales score ≤30. siRNAs were designed as 19-bp RNA duplexes with an asymmetric 3′ overhang at the 3′ end of the antisense strand. We tested if these siRNAs effectively reduced tyrosinase gene expression using qRT-PCR and found that 17 siRNA sequences were more effective than commercially available siRNA. Three siRNAs further tested showed an effective visual color change in MNT-1 human cells without cytotoxic effects, indicating these sequences are anti-melanogenic. Our study revealed that human tyrosinase siRNAs could be efficiently designed using multiple prediction algorithms.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Tirosina / Pesos y Medidas / ARN / Pigmentación de la Piel / Dihidroxifenilalanina / Expresión Génica / Monofenol Monooxigenasa / ARN Interferente Pequeño / Interferencia de ARN / Melaninas Tipo de estudio: Estudio pronóstico Límite: Humanos Idioma: Inglés Revista: Biomolecules & Therapeutics Año: 2018 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Tirosina / Pesos y Medidas / ARN / Pigmentación de la Piel / Dihidroxifenilalanina / Expresión Génica / Monofenol Monooxigenasa / ARN Interferente Pequeño / Interferencia de ARN / Melaninas Tipo de estudio: Estudio pronóstico Límite: Humanos Idioma: Inglés Revista: Biomolecules & Therapeutics Año: 2018 Tipo del documento: Artículo