Patterns of rpoC Mutations in Drug-Resistant Mycobacterium tuberculosis Isolated from Patients in South Korea / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
;
: 222-227, 2018.
Artículo
en Inglés
| WPRIM
| ID: wpr-715740
ABSTRACT
BACKGROUND:
Rifampicin (RFP) is one of the principal first-line drugs used in combination chemotherapies against Mycobacterium tuberculosis, and its use has greatly shortened the duration of chemotherapy for the successful treatment of drug-susceptible tuberculosis. Compensatory mutations have been identified in rpoC that restore the fitness of RFP-resistant M. tuberculosis strains with mutations in rpoB. To investigate rpoC mutation patterns, we analyzed 93 clinical M. tuberculosis isolates from patients in South Korea.METHODS:
Drug-resistant mycobacterial isolates were cultured to determine their susceptibility to anti-tubercular agents. Mutations in rpoC were identified by sequencing and compared with the relevant wild-type DNA sequence.RESULTS:
In total, 93 M. tuberculosis clinical isolates were successfully cultured and tested for drug susceptibilities. They included 75 drug-resistant tuberculosis species, of which 66 were RFP-resistant strains. rpoC mutations were found in 24 of the 66 RFP-resistant isolates (36.4%). Fifteen different types of mutations, including single mutations (22/24, 91.7%) and multiple mutations (2/24, 8.3%), were identified, and 12 of these mutations are reported for the first time in this study. The most frequent mutation involved a substitution at codon 452 (nt 1356) resulting in amino acid change F452L.CONCLUSION:
Fifteen different types of mutations were identified and were predominantly single-nucleotide substitutions (91.7%). Mutations were found only in dual isoniazid- and RFP-resistant isolates of M. tuberculosis. No mutations were identified in any of the drug-susceptible strains.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Rifampin
/
Tuberculosis
/
Codón
/
Secuencia de Bases
/
Tuberculosis Resistente a Múltiples Medicamentos
/
Resistencia a Múltiples Medicamentos
/
Quimioterapia
/
Quimioterapia Combinada
/
Corea (Geográfico)
/
Mycobacterium
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Tuberculosis and Respiratory Diseases
Año:
2018
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS