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Identification of Fastidious Mycobacteria other than M. tuberculosis ( MOTT ) by Comparative Sequence Analysis of rpoB and 16S rDNA
Journal of Bacteriology and Virology ; : 39-45, 2002.
Artículo en Coreano | WPRIM | ID: wpr-71648
ABSTRACT
Conventional tests for the identification of mycobacteria may frequently result in erroneous identification and underestimate the diversity within the genus Mycobacterium. However, this problem can be overcome by molecular approach like as 16S rRNA gene (rDNA) or RNA polymerase gene (rpoB) sequence analysis. In this study, a molecular approach analyzing partial sequence of 16S rDNA and rpoB gene was applied to mycobacteria other than M tuberculosis (MOTT) isolates that had not been definitely identified by conventional physical and biochemical tests. Among the eighteen isolates included in this study, twelve isolates could be identified to the species level and six were identified to the complex level. Compared with the results by 16S rDNA analysis, the rpoB analysis could di6erentiate some of the strains into the subspecies level.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Tuberculosis / ARN Polimerasas Dirigidas por ADN / ADN Ribosómico / Análisis de Secuencia / Genes de ARNr / Mycobacterium Tipo de estudio: Estudio diagnóstico Idioma: Coreano Revista: Journal of Bacteriology and Virology Año: 2002 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Tuberculosis / ARN Polimerasas Dirigidas por ADN / ADN Ribosómico / Análisis de Secuencia / Genes de ARNr / Mycobacterium Tipo de estudio: Estudio diagnóstico Idioma: Coreano Revista: Journal of Bacteriology and Virology Año: 2002 Tipo del documento: Artículo