IgE Cross-Reactivity between Humulus japonicus and Humulus lupulus
Yonsei Medical Journal
;
: 852-856, 2018.
Artículo
en Inglés
| WPRIM
| ID: wpr-716927
ABSTRACT
PURPOSE:
Japanese hop (Humulus japonicus) is a major cause of weed pollinosis in East Asia. However, supplies of commercial allergen extract from this plant have not met clinical demand. The pollen of common hop (Humulus lupulus), a closely related species, may provide an alternative source if there is strong IgE cross-reactivity between these two species. We aimed to compare the IgE cross-reactivity and allergenicity of common hop and Japanese hop pollen. MATERIALS ANDMETHODS:
Cross-reactivity was measured by inhibition ELISA. One- and two-dimensional (2D) gel analyses combined with IgE immunoblotting and mass spectrometry [liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)] were performed to detect IgE-reactive pollen components.RESULTS:
Up to 16.7% of IgE reactivity to Japanese hop was inhibited by common hop. A 12-kDa protein component of Japanese hop pollen that showed the most potent IgE reaction was absent from common hop. Six IgE-reactive components from Japanese hop were detected by 2D gel electrophoresis and LC-ESI-MS/MS, but showed low Mascot scores, preventing positive identification.CONCLUSION:
No significant IgE cross-reaction was observed for Japanese and common hop pollen allergens. Development of allergy diagnostic and immunotherapeutic reagents based on Japanese hop pollen are urgently needed.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plantas
/
Polen
/
Espectrometría de Masas
/
Inmunoglobulina E
/
Ensayo de Inmunoadsorción Enzimática
/
Alérgenos
/
Electroforesis en Gel Bidimensional
/
Immunoblotting
/
Rinitis Alérgica Estacional
/
Cromatografía
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
2018
Tipo del documento:
Artículo
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