Your browser doesn't support javascript.
loading
Detection of Rifampicin Resistance in Mycobacterium tuberculosis by Using Middlebrook 7H9 Broth Medium with 2,3-Diphenyl-5-Thienyl-(2)-Tetrazolium Chloride / 대한임상미생물학회지
Annals of Clinical Microbiology ; : 47-50, 2018.
Artículo en Inglés | WPRIM | ID: wpr-717233
ABSTRACT

BACKGROUND:

A simple and cost-effective method is needed for the detection of rifampicin resistance in Mycobacterium tuberculosis in resource-limited settings. We suggest a broth medium-based method using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride (STC) for detection of rifampin resistance of tubercle bacilli within a reasonable time frame.

METHODS:

The type strain (M. tuberculosis H37Rv) and 45 cultured clinical strains of M. tuberculosis (35 rifampin-susceptible and 10 rifampin-resistant) were used. Phenotypes of rifampicin resistance were tested by the Korea Institute of Tuberculosis, and confirmed by GenoType MTBDRplus (Hain Lifescience, Germany). Susceptibility tests were performed using STC-containing OADC-enriched Middlebrook 7H9 broth (BD, USA).

RESULTS:

All tests were finished in 3 to 6 days. The same results were obtained with the standard and current methods for all 45 clinical isolates (100% sensitivity and specificity for resistance detection).

CONCLUSION:

The current method using STC is a good alternative for detecting M. tuberculosis rifampin resistance in a cost-effective and timely fashion, which is particularly important in resource-limited settings.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fenotipo / Rifampin / Tuberculosis / Sensibilidad y Especificidad / Genotipo / Corea (Geográfico) / Métodos / Mycobacterium / Mycobacterium tuberculosis Tipo de estudio: Estudio diagnóstico País/Región como asunto: Asia Idioma: Inglés Revista: Annals of Clinical Microbiology Año: 2018 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fenotipo / Rifampin / Tuberculosis / Sensibilidad y Especificidad / Genotipo / Corea (Geográfico) / Métodos / Mycobacterium / Mycobacterium tuberculosis Tipo de estudio: Estudio diagnóstico País/Región como asunto: Asia Idioma: Inglés Revista: Annals of Clinical Microbiology Año: 2018 Tipo del documento: Artículo