BubR1 Insufficiency Impairs Affective Behavior and Memory Function in Mice / 대한배뇨장애요실금학회지
International Neurourology Journal
;
: S122-S130, 2018.
Artículo
en Inglés
| WPRIM
| ID: wpr-717679
ABSTRACT
PURPOSE:
Although aging causes functional declines in cognition, the molecular mechanism underlying these declines remains largely unknown. Recently, the spindle checkpoint kinase budding uninhibited by benzimidazole-related 1 (BubR1) has emerged as a key determinant for age-related pathology in various tissues including brain. However, the neurobehavioral impact of BubR1 has not been explored. In this study, we investigated the role of BubR1 in behavioral function.METHODS:
To investigate the neurobiological functions of BubR1 in vivo, we utilized transgenic mice harboring BubR1 hypomorphic alleles (BubR1 H/H mice), which produce low amounts of BubR1 protein, as well as mice that have specific knockdown of BubR1 in the adult dentate gyrus. To assess anxiety-like behavior, the above groups were subjected to the elevated plus maze and the light-dark test, in addition to utilizing the tail-suspension and forced-swim test to determine depression-like behavior. We used novel object recognition to test for memory-related function.RESULTS:
We found that BubR1 H/H mice display several behavioral deficits when compared to wild-type littermates, including increased anxiety in the elevated-plus maze test, depression-like behavior in the tail suspension test, as well as impaired memory function in the novel object recognition test. Similar to BubR1 H/H mice, knockdown of BubR1 within the adult dentate gyrus led to increased anxiety-like behavior as well as depression-like behavior, and impaired memory function.CONCLUSIONS:
Our study demonstrates a requirement of BubR1 in maintaining proper affective and memory-related behavioral function. These results suggest that a decline in BubR1 levels with advanced age may be a crucial contributor to age-related hippocampal dysfunction.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ansiedad
/
Patología
/
Fosfotransferasas
/
Encéfalo
/
Envejecimiento
/
Ratones Transgénicos
/
Cognición
/
Giro Dentado
/
Suspensión Trasera
/
Alelos
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
International Neurourology Journal
Año:
2018
Tipo del documento:
Artículo
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