Prolonged low-dose cyclophosphamide treatment after pulse therapy attenuates lung injury in rats with paraquat intoxication
The Korean Journal of Internal Medicine
;
: 1137-1142, 2018.
Artículo
en Inglés
| WPRIM
| ID: wpr-718018
ABSTRACT
BACKGROUND/AIMS:
This study tested the hypothesis that prolonged low-dose cyclophosphamide (CTX) treatment after pulse therapy attenuate paraquat (PQ)-induced lung injury in rats.METHODS:
PQ (25 mg/kg) was administered intraperitoneally to induce PQ-intoxicated rat model. The rats were randomly divided into four groups control group (1 mL/day saline solution for 14 days), PQ group (1 mL/day saline solution for 14 days after PQ exposure), pulse group (15 mg/kg/day CTX in 1 mL of saline solution for 2 days and subsequent 1 mL/day saline solution for 12 days), and prolonged low-dose group (15 mg/kg/day CTX in 1 mL of saline solution for 2 days and subsequent 1.5 mg/kg/day CTX in 1 mL of saline solution for 12 days). A 14-day follow-up was conducted to determine the survival rat, and lung hydroxyproline (HYP), wet-to-dry weight ratios (W/Dc) and histopathological changes were evaluated.RESULTS:
Results showed similar survival rate (55% vs. 50%, p > 0.05) between prolonged low-dose and pulse groups. Lung W/Dc (4.94 ± 0.38 vs. 5.47 ± 0.28, p < 0.01), HYP (3.34 ± 0.29 µg/mg vs. 3.65 ± 0.19 µg/mg, p < 0.001), and fibrosis score (2.69 ± 0.84 vs. 3.13 ± 0.63, p < 0.05) were lower in prolonged low-dose group than those in the pulse group.CONCLUSIONS:
These findings suggested prolonged low-dose CTX treatment after pulse therapy could attenuate PQ-induced lung injury in rats.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Paraquat
/
Fibrosis
/
Cloruro de Sodio
/
Tasa de Supervivencia
/
Estudios de Seguimiento
/
Ciclofosfamida
/
Modelos Animales
/
Lesión Pulmonar
/
Hidroxiprolina
/
Pulmón
Tipo de estudio:
Estudio observacional
/
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
The Korean Journal of Internal Medicine
Año:
2018
Tipo del documento:
Artículo
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