Characterization of Brachyspira hyodysenteriae isolates from Korea
Journal of Veterinary Science
;
: 335-339, 2005.
Artículo
en Inglés
| WPRIM
| ID: wpr-71817
ABSTRACT
This study was done to characterize diversity in 10 Brachyspira hyodysenteriae isolates in Korea. The isolates were compared with 14 well-characterized non-Korean strains of various Brachyspira species. All Korean isolates showed strong beta haemolysis and had blunt cell ends with 7~14 periplasmic flagella. They produced indole, and did not ferment fructose. They were alpha-glucosidase positive and alpha-galatosidase negative using the APIZYM kit. Using polyclonal antisera raised in rabbits against recognized serotypes, all isolates showed a strong reaction to B. hyodysenteriae antisera E, A and B. Using multilocus enzyme electrophoresis (MLEE) with 15 enzymes and 5 buffer systems, the Korean and non-Korean isolates were divided into 22 electrophoretic types (ETs) and 5 divisions (A, B, C, D and E). Division A corresponded to B. hyodysenteriae, B to B. innocens, C to B. intermedia, D to B. murdochii and E to B. pilosicoli. The 10 Korean isolates of B. hyodysenteriae were relatively diverse, being divided into 9 ETs within MLEE division A. They were all distinct from the non-Korean strains.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Infecciones por Spirochaetales
/
Porcinos
/
Enfermedades de los Porcinos
/
Variación Genética
/
Serotipificación
/
Brachyspira hyodysenteriae
/
Electroforesis
/
Genes Bacterianos
/
Corea (Geográfico)
Límite:
Animales
País/Región como asunto:
Asia
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2005
Tipo del documento:
Artículo
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