Comparison of PANA RealTyper HPV Kit with AdvanSure HPV GenoBlot Assay for Human Papillomavirus Genotyping / 대한임상미생물학회지
Annals of Clinical Microbiology
;
: 86-91, 2018.
Artículo
en Coreano
| WPRIM
| ID: wpr-718743
ABSTRACT
BACKGROUND:
The PANA RealTyper HPV kit (PANAGENE, Korea; PANA RealTyper) was developed to genotype human papillomavirus (HPV) and was based on multiplex real-time PCR amplification and melting curve analysis. In this study, we compared PANA RealTyper to the AdvanSure HPV GenoBlot assay (LG Life Sciences, Korea; AdvanSure assay) and attempted to evaluate the performance of PANA RealTyper.METHODS:
A total of 60 cervical specimens were collected from women undergoing routine cervical cancer screening. The AdvanSure assay and PANA RealTyper kit identified the same 20 high-risk genotypes. However, the AdvanSure assay identified 15 low-risk genotypes, while the PANA RealTyper kit identified only 2 but detected 18 low-risk genotypes.RESULTS:
Among the total 60 specimens, 54 high-risk genotypes (40 specimens) and 20 low-risk genotypes (18 specimens) were detected. The agreement rates of the assays ranged from 94.4 to 100% for high-risk genotypes. Among 9 genotypes that were positive in the PANA RealTyper kit but negative in the AdvanSure assay, 7 were confirmed as true positive (HPV genotypes 16 (n=1), 39 (n=1), 52 (n=1), 58 (n=2), 68 (n=2)). Among 4 genotypes that were negative in the PANA RealTyper kit but positive in the AdvanSure assay, 3 were confirmed as HPV genotype 59. Among the 19 low-risk genotypes positive in the AdvanSure assay, there were 2 cases of HPV 6 and 1 case of HPV 11. In comparison, only 1 positive case of HPV 6 was determined by the PANA RealTyper kit.CONCLUSION:
The PANA RealTyper kit was comparable with the AdvanSure assay. The PANA RealTyper kit would be useful and suitable for HPV genotyping in the clinical laboratory.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neoplasias del Cuello Uterino
/
Tamizaje Masivo
/
Disciplinas de las Ciencias Biológicas
/
Papillomavirus Humano 6
/
Papillomavirus Humano 11
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Congelación
/
Genotipo
/
Corea (Geográfico)
Tipo de estudio:
Estudio de tamizaje
Límite:
Femenino
/
Humanos
País/Región como asunto:
Asia
Idioma:
Coreano
Revista:
Annals of Clinical Microbiology
Año:
2018
Tipo del documento:
Artículo
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