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Nucleoprotein vaccine induces cross-protective cytotoxic T lymphocytes against both lineages of influenza B virus
Clinical and Experimental Vaccine Research ; : 54-63, 2019.
Artículo en Inglés | WPRIM | ID: wpr-719487
ABSTRACT

PURPOSE:

The influenza B virus diverges into two antigenically distinct lineages B/Yamagata and B/Victoria. Influenza B is the dominant circulating virus during some influenza seasons, and recent data demonstrated that influenza A and B infection similarly cause severe clinical symptoms in hospitalized patients. Nucleoprotein (NP) is a good target for a universal influenza vaccine. This study investigated whether NP epitope variation within two lineages affects the dominant cytotoxic T lymphocyte (CTL) responses induced by vaccination and the resultant protective immunity. MATERIALS AND

METHODS:

The NP of B/Yamagata/16/1988, the representative strain of the Yamagata lineage, includes a dominant CTL epitope, FSPIRITFL, while B/Shangdong/7/1997 from the Victoria lineage has one amino acid difference in this sequence, FSPIRVTFL. Two recombinant replication-deficient adenovirus (rAd)-vectored vaccines expressing either NP were prepared (rAd/B-NP(I) and rAd/B-NP(V), respectively) and administered to BALB/c mice intranasally. To examine the efficacy of vaccination, antibody responses, CTL responses, and morbidity/mortality after challenge were measured.

RESULTS:

Both vaccines induce similar antibody and CD8 T-cell responses cross-reacting to both epitopes, and also confer cross-protection against both lineages regardless of amino acid difference.

CONCLUSION:

The rAd-vectored vaccine expressing the NP could be developed as universal influenza B vaccine which provides broader protection.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Virus de la Influenza B / Estaciones del Año / Victoria / Vacunas contra la Influenza / Vacunas / Linfocitos / Linfocitos T / Linfocitos T Citotóxicos / Adenoviridae / Vacunación Límite: Animales / Humanos País/Región como asunto: Oceanía Idioma: Inglés Revista: Clinical and Experimental Vaccine Research Año: 2019 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Virus de la Influenza B / Estaciones del Año / Victoria / Vacunas contra la Influenza / Vacunas / Linfocitos / Linfocitos T / Linfocitos T Citotóxicos / Adenoviridae / Vacunación Límite: Animales / Humanos País/Región como asunto: Oceanía Idioma: Inglés Revista: Clinical and Experimental Vaccine Research Año: 2019 Tipo del documento: Artículo