The Characteristics of Residual Leukocyte in Sepacell PLS-5A Filtered Platelet Concentrates / 대한혈액학회지
Korean Journal of Hematology
;
: 406-410, 1998.
Artículo
en Coreano
| WPRIM
| ID: wpr-720414
ABSTRACT
BACKGROUND:
We evaluated residual leukocytes characteristics of white cell(WBC) reduction filter in platelet concentrates. Differential count and lymphocyte subset changes were measured before and after leukocyte filtration in platelet concentrates. MATERIAL ANDMETHODS:
Ten units of platelet concentrates were prepared and were filtered with WBC-reduction filter(Sepacell PLS 5A, Japan). After filtration of blood products, WBC and differential leukocyte count and lymphocyte subsets were counted by microscopic examination of Wright-Giemsa stained smear and Facscan(Becton-Dickinson, USA). Monoclonal antibodies used for lymphocyte subset test were CD3(FITC), CD4(FITC), CD8(PE), CDl4(PE), CDl6(PE), CDl9(PE), CD33(PE), CD56(PE), IgGl(FITC), IgG2(PE).RESULTS:
The main population of residual leukocytes after filtration was mainly lymphocytes(96.7%), and CD3 positive T lymphocytes showed 23.8% positivity of residual leukocyctes and the next were NK cell(8.7%). B lymphocytes were rarely found(<0.01%) and CD4/ CD8 ratio was within normal limits.CONCLUSION:
The leukocyte reduction filters(Sepacell PLS-5A) would be effective for prevention of platelet alloimmunization but not sure about the effect for prevention of TA GVHD.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plaquetas
/
Linfocitos B
/
Linfocitos T
/
Subgrupos Linfocitarios
/
Filtración
/
Recuento de Leucocitos
/
Leucocitos
/
Anticuerpos Monoclonales
Idioma:
Coreano
Revista:
Korean Journal of Hematology
Año:
1998
Tipo del documento:
Artículo
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