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Eugenol Induces a Reactive Oxygen Species-mediated Apoptosis in HL-60 Human Promyelocytic Leukemia Cells
Korean Journal of Hematology ; : 65-74, 2005.
Artículo en Coreano | WPRIM | ID: wpr-720445
ABSTRACT

BACKGROUND:

Eugenol is a major component of the essential oil isolated from Eugenia caryophyllata (Myrtaceae), and has been widely used as a traditional medicine. In this study, the effects of eugenol on the cytotoxicity, induction of apoptosis and putative pathways of its actions were investigated in human promyelocytic leukemia cells (HL-60).

METHODS:

After applying eugenol to cultured HL-60, the changes in the mitochondrial membrane potential of the cells were monitored after double staining with propidium iodide and rhodamine 123, with 2', 7'-dicholorofluorescin diacetate was used to measure of levels of reactive oxygen species (ROS)

RESULTS:

Eugenol was shown to be a potent inducer of apoptosis; transducing the apoptotic signal via ROS generation; thereby, inducing mitochondrial permeability transition (MPT) and cytochrome c release to the cytosol. The production of ROS, mitochondrial alteration and subsequent apoptotic cell death in eugenol-treated cells were blocked by the antioxidant, N-acetylcystein (NAC).

CONCLUSION:

Taken together, the present study has demonstrated that eugenol induces ROS-mediated mitochondrial permeability transition and resultant cytochrome c release.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Oxígeno / Permeabilidad / Propidio / Eugenol / Leucemia / Muerte Celular / Especies Reactivas de Oxígeno / Apoptosis / Células HL-60 / Rodamina 123 Límite: Humanos Idioma: Coreano Revista: Korean Journal of Hematology Año: 2005 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Oxígeno / Permeabilidad / Propidio / Eugenol / Leucemia / Muerte Celular / Especies Reactivas de Oxígeno / Apoptosis / Células HL-60 / Rodamina 123 Límite: Humanos Idioma: Coreano Revista: Korean Journal of Hematology Año: 2005 Tipo del documento: Artículo