Pattern of Telomerase Activities in Acute Leukemias and Chronic Myeloid Leukemia / 대한혈액학회지
Korean Journal of Hematology
;
: 189-196, 2001.
Artículo
en Coreano
| WPRIM
| ID: wpr-720533
ABSTRACT
BACKGROUND:
Telomerase has a leading role as a potential enzyme responsible for tumorigenesis and longevity. Telomerase is a ribonucleoprotein that synthesizes a specific repeating nucleotide sequence onto the ends of telomeres. This enzyme is normally present in immortalized cell lines, germ-line tissues, and tumor tissues. We intended to compare the telomerase activity among various types of leukemia to determine the association of telomerase activity and patients status and responsiveness of chemotherapy.METHODS:
Specimens were collected from Jan. 1999 to Oct. 1999 and included the leukemic bone marrow (ALL, AML and CML) and the peripheral blood or bone marrow of normal persons or patients with iron deficiency anemia and immune thrombocytopenic purpura. Telomerase activity was measured by TRAP assay using Telomerase PCR ELISA kit.RESULTS:
Telomerase activities were increased in acute leukemias and relapsed acute leukemia cases, whereas in the cases of complete remission state of acute leukemia, the activity was decreased. Telomerase activity was increased in leukemias which had high percentage of immature cells, especially more than 70% of blast. Also the activity was decreased in post-chemotherapeutic group, whereas increased in untreated group. There was no significant difference between prognosis of chromosomal abnormalities and telomerase activity.CONCLUSION:
These results showed that telomerase activity was increased in acute phase of leukemia, high percentage of immature cells, and chemoresistant group of leukemia.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Pronóstico
/
Ribonucleoproteínas
/
Médula Ósea
/
Ensayo de Inmunoadsorción Enzimática
/
Secuencia de Bases
/
Leucemia Mielógena Crónica BCR-ABL Positiva
/
Leucemia
/
Línea Celular
/
Reacción en Cadena de la Polimerasa
/
Aberraciones Cromosómicas
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Hematology
Año:
2001
Tipo del documento:
Artículo
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