Immunoreactivity of Iron-Repressible Cell Wall Proteins and Exoproteins of Staphylococcus aureus / 감염과화학요법
Infection and Chemotherapy
; : 241-244, 2004.
Article
en En
| WPRIM
| ID: wpr-721784
Biblioteca responsable:
WPRO
ABSTRACT
In the present study, we tried to investigate immunoreactivity of iron-repressible cell wall proteins and exoproteins of Staphylococcus aureus when the convalescent serum obtained from the patient with septicemia was applied. On SDS-PAGE and western blot analysis, several iron-repressible cell wall proteins expressed in iron-deficient BHI showed strong immunoreactivity, whereas no or relatively weak immunoreactivity was shown in iron-sufficient BHI. Several exoproteins were expressed only in iron-deficient BHI and these exoproteins showed strong immunoreactivity. These results indicate that several iron-repressible cell wall proteins and exoproteins are expressed and are immunogenic in vivo. Since in vivo state is an relatively iron-restricted condition, we recommend the use of iron-deficient media for studies concerning pathogenicity of staphylococcal in human.
Texto completo:
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Índice:
WPRIM
Asunto principal:
Staphylococcus
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Staphylococcus aureus
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Virulencia
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Pared Celular
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Western Blotting
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Sepsis
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Electroforesis en Gel de Poliacrilamida
Límite:
Humans
Idioma:
En
Revista:
Infection and Chemotherapy
Año:
2004
Tipo del documento:
Article