Use of RNA/RNA Duplex, Base Pair-mismatch Assay and 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl Tetrazolium Bromide (MTT) Assay for Rapid Detection of Rifampin-resistant Mycobacterium tuberculosis / 감염과화학요법
Infection and Chemotherapy
;
: 12-17, 2003.
Artículo
en Coreano
| WPRIM
| ID: wpr-722222
ABSTRACT
BACKGROUND:
Multidrug-resistant tuberculosis is an increasing concern for public health in many parts of the world. We have evaluated the specificity and sensitivity of the mismatch assay and 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay in detecting rifampin- resistant Mycobacterium tuberculosis.METHODS:
Eleven rifampin-susceptible and 15 rifampin-resistant M. tuberculosis strains were isolated from clinical specimens obtained from patients in Yonsei University College of Medicine, Severance Hospital. RNA/RNA duplex, base pair-mismatch assay (Mismatch Detect II kit, Ambion) and MTT assay were performed.RESULTS:
The specificity and sensitivity of detection of rifampin resistance were 91% and 87% in mismatch assay and 73% and 67% in MTT assay, respectively.CONCLUSION:
These results suggest the usefulness of mismatch assay in detecting rifampin-resistant Mycobacterium tuberculosis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Rifampin
/
Tuberculosis
/
Salud Pública
/
Sensibilidad y Especificidad
/
Tuberculosis Resistente a Múltiples Medicamentos
/
Mycobacterium
/
Mycobacterium tuberculosis
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Coreano
Revista:
Infection and Chemotherapy
Año:
2003
Tipo del documento:
Artículo
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