The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Journal of Veterinary Science
; : 277-280, 2006.
Article
en En
| WPRIM
| ID: wpr-72554
Biblioteca responsable:
WPRO
ABSTRACT
Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently, B. hyodysenteriae and B. pilosicoli revealed different restriction patterns; however, the other three species shared the same pattern. These findings are not consistent with a prior report. Differences in 23S rRNA gene sequences, between two B. murdochii strains, 56-150 and 155-20, were observed. These results indicate that 23S rRNA PCR-RFLP could be used as an identification method for pathogenic Brachyspira spp. (B. hyodysenteriae and B. pilosicoli) as well as an epidemiological tool for characterizing spirochetes isolated from swine.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Filogenia
/
Spirochaetales
/
Infecciones por Spirochaetales
/
Porcinos
/
Enfermedades de los Porcinos
/
Polimorfismo de Longitud del Fragmento de Restricción
/
ADN Bacteriano
/
ARN Ribosómico 23S
/
Reacción en Cadena de la Polimerasa
/
Disentería Bacilar
Tipo de estudio:
Prognostic_studies
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Journal of Veterinary Science
Año:
2006
Tipo del documento:
Article