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Caffeine Induces Apoptosis in Human Neuroblastoma Cell Line SK-N-MC
Journal of Korean Medical Science ; : 674-678, 2002.
Artículo en Inglés | WPRIM | ID: wpr-72660
ABSTRACT
Caffeine is one of the most widely consumed neuroactive drugs, coming mostly from everyday beverages such as coffee and tea. To investigate whether caffeine induces apoptosis in the central nervous system, 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay, 4,6-diamidino-2-phenylindole (DAPI) staining, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, flow cytometric analysis, DNA fragmentation assay, and caspase-3 enzyme assay were performed on SK-N-MC human neuroblastoma cells. Cells treated with caffeine at concentrations as high as 10 mM exhibited several characteristics of apoptosis. In addition, caffeine was shown to increase the caspase-3 activity. These results suggest that high-dose of caffeine induces apoptosis in human neuroblastoma cells, probably by increasing the caspase-3 enzyme activity.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Cafeína / Células Tumorales Cultivadas / Ciclo Celular / Supervivencia Celular / Sistema Nervioso Central / Apoptosis / Caspasas / Caspasa 3 / Fragmentación del ADN / Neuroblastoma Límite: Humanos Idioma: Inglés Revista: Journal of Korean Medical Science Año: 2002 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Cafeína / Células Tumorales Cultivadas / Ciclo Celular / Supervivencia Celular / Sistema Nervioso Central / Apoptosis / Caspasas / Caspasa 3 / Fragmentación del ADN / Neuroblastoma Límite: Humanos Idioma: Inglés Revista: Journal of Korean Medical Science Año: 2002 Tipo del documento: Artículo