Potassium Currents in Isolated Deiters' Cells of Guinea Pig
The Korean Journal of Physiology and Pharmacology
;
: 537-546, 2013.
Artículo
en Inglés
| WPRIM
| ID: wpr-727604
ABSTRACT
Deiters' cells are the supporting cells in organ of Corti and are suggested to play an important role in biochemical and mechanical modulation of outer hair cells. We successfully isolated functionally different K+ currents from Deiters' cells of guinea pig using whole cell patch clamp technique. With high K+ pipette solution, depolarizing step pulses activated strongly outward rectifying currents which were dose-dependently blocked by clofilium, a class III anti-arrhythmic K+ channel blocker. The remaining outward current was transient in time course whereas the clofilium-sensitive outward current showed slow inactivation and delayed rectification. Addition of 5 mM tetraethylammonium (TEA) further blocked the remaining current leaving a very fast inactivating transient outward current. Therefore, at least three different types of K+ current were identified in Deiters' cells, such as fast activating and fast inactivating current, fast activating slow inactivating current, and very fast inactivating transient outward current. Physiological role of them needs to be established.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Órgano Espiral
/
Farmacología
/
Potasio
/
Canales de Potasio
/
Guinea
/
Tetraetilamonio
/
Cobayas
/
Cabello
/
Audición
/
Compuestos de Amonio Cuaternario
Límite:
Animales
País/Región como asunto:
Africa
Idioma:
Inglés
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2013
Tipo del documento:
Artículo
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