Inhibition by higenamine of lipopolysaccharide-induced iNOS and mRNA expression and NO production in rat aorta
The Korean Journal of Physiology and Pharmacology
;
: 297-302, 1997.
Artículo
en Inglés
| WPRIM
| ID: wpr-727640
ABSTRACT
Higenamine was widely used as traditional remedy for the treatment of rheumatoid arthritis. Nitric oxide (NO) may be a critical mediator in this inflammatory disease. Synovial tissue from humans with inflammatory arthritis expresses NOS2 (iNOS) mRNA and protein, and generates NO in vitro. We therefore, investigated the effect of higenamine on the induction of nitric oxide synthase (NOS) promoted by lipopolysaccharide (LPS). Prophylactic application of higenamine selectively prevented LPS-primed initiation of L-arginine-induced relaxation and restored phenylephrine(PE)-induced contraction in rat aorta. LPS-stimulated nitrite production in the incubation medium was reduced by higenamine. Furthermore, RT-PCR and Northern analysis indicated that higenamine reduced iNOS expression primed by LPS in rat aorta. These results suggest that higenamine prevents LPS-promoted induction of NOS in vascular smooth muscle.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Aorta
/
Artritis
/
Artritis Reumatoide
/
Relajación
/
ARN Mensajero
/
Óxido Nítrico Sintasa
/
Músculo Liso Vascular
/
Óxido Nítrico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
1997
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS