Effects of cholecystokinin octapeptide on neuronal activities in the rat nucleus tractus solitarius
The Korean Journal of Physiology and Pharmacology
;
: 275-281, 2000.
Artículo
en Inglés
| WPRIM
| ID: wpr-727729
ABSTRACT
Cholecystokinin (CCK) is a gastrointestinal hormone which plays an important role in satiety and gastric motility. It is also widely distributed throughout the central nervous system, where it appears to be involved in the central control of anxiety, feeding behavior and nociception. Two distinct CCK receptor types, CCKA and CCKB, have been found in the brain. Both CCK receptors coexist in the rat nucleus tractus solitarius (NTS), which is the primary center for the coordination of peripheral and central activities related to gastrointestinal, cardiovascular and respiratory functions. In order to study ionic actions of CCK on each type of receptor, we investigated the effects of CCK-8S on neurons located in the NTS of the rat using whole-cell patch-clamp recordings in brainstem slices. Application of CCK-8S, under current clamp, produced a membrane depolarization accompanied by action potential firing. This CCK-evoked excitation was dose-dependent (10 nM ~ 10 micrometer) and observed in more than 60% of NTS neurons. Under voltage clamp conditions, CCK-8S induced an inward current with a notably increased spontaneous excitatory synaptic activity. However, CCK-8S did not significantly change the amplitude of pharmacologically isolated and evoked EPSP(C)s. Using selective CCKA and CCKB receptor antagonists, we observed two different effects of CCK-8S, which suggest CCKA receptor-mediated inhibitory and CCKB receptor-mediated excitatory effects in the NTS. These results may help to explain the ability of CCK to modulate gastrointestinal and other reflex systems in the NTS.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ansiedad
/
Reflejo
/
Sincalida
/
Encéfalo
/
Tronco Encefálico
/
Potenciales de Acción
/
Colecistoquinina
/
Sistema Nervioso Central
/
Receptores de Colecistoquinina
/
Núcleo Solitario
Límite:
Animales
Idioma:
Inglés
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2000
Tipo del documento:
Artículo
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