Role of phospholipase A2 in oxidant-induced alteration in phosphate transport in primary cultured rabbit renal proximal tubule cells
The Korean Journal of Physiology and Pharmacology
;
: 601-609, 1998.
Artículo
en Inglés
| WPRIM
| ID: wpr-727756
ABSTRACT
The present study was undertaken to examine the role of phospholipase A2 (PLA2) in oxidant-induced inhibition of phosphate transport in primary cultured rabbit renal proximal tubule cells. Uptakes of phosphate and glucose were dose-dependently inhibited by an oxidant t-butylhydroperoxide (tBHP), and the significant inhibition appeared at 0.025 mM of tBHP, whereas tBHP-induced alterations in lipid peroxidation and cell viability were seen at 0.5 mM. tBHP stimulated arachidonic acid (AA) release in a dose-dependent fashion. A PLA2 inhibitor mepacrine prevented tBHP-induced AA release, but it did not alter the inhibition of phosphate uptake and the decrease in cell viability induced by tBHP. tBHP-induced inhibition of phosphate transport was not affected by a PKC inhibitor, staurosporine. tBHP at 0.1 mM did not produce the inhibition of Na+-K+-ATPase activity in microsomal fraction, although it significantly inhibited at 1.0 mM. These results suggest that tBHP can inhibit phosphate uptake through a mechanism independent of PLA2 activation, irreversible cell injury, and lipid peroxidation in primary cultured rabbit renal proximal tubular cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosfolipasas
/
Quinacrina
/
Peroxidación de Lípido
/
Supervivencia Celular
/
Ácido Araquidónico
/
Estaurosporina
/
Terc-Butilhidroperóxido
/
Fosfolipasas A2
/
Glucosa
Idioma:
Inglés
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
1998
Tipo del documento:
Artículo
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