Identification of Differentially Expressed Genes in Bovine Follicular Cystic Ovaries
The Korean Journal of Physiology and Pharmacology
;
: 265-272, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-727790
ABSTRACT
Follicular cystic ovary (FCO) is one of the most frequently diagnosed ovarian diseases and is a major cause of reproductive failure in mammalian species. However, the mechanism by which FCO is induced remains unclear. Genetic alterations which affect the functioning of many kinds of cells and/or tissues could be present in cystic ovaries. In this study, we performed a comparison analysis of gene expression in order to identify new molecules useful in discrimination of bovine FCO with follicular cystic follicles (FCFs). Normal follicles and FCFs were classified based on their sizes (5 to 10 mm and > or =25 mm). These follicles had granulosa cell layer and theca interna and the hormone 17beta-estradiol (E2)/ progesterone (P4) ratio in follicles was greater than one. Perifollicular regions including follicles were used for the preparation of RNA or protein. Differentially expressed genes (DEG) that showed greater than a 2-fold change in expression were screened by the annealing control primer (ACP)-based PCR method using GeneFishing(TM) DEG kits in bovine normal follicles and FCFs. We identified two DEGs in the FCFs ribosomal protein L15 (RPL15) and microtubule-associated protein 1B (MAP1B) based on BLAST searches of the NCBI GenBank. Consistent with the ACP analysis, semi-quantitative PCR data and Western blot analyses revealed an up-regulation of RPL15 and a down-regulation of MAP1B in FCFs. These results suggest that RPL15 and MAP1B may be involved in the regulation of pathological processes in bovine FCOs and may help to establish a bovine gene data-base for the discrimination of FCOs from normal ovaries.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Enfermedades del Ovario
/
Ovario
/
Procesos Patológicos
/
Progesterona
/
Proteínas Ribosómicas
/
Células Tecales
/
Quiste Folicular
/
ARN
/
Regulación hacia Abajo
/
Expresión Génica
Tipo de estudio:
Estudio diagnóstico
Idioma:
Inglés
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2010
Tipo del documento:
Artículo
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