Toll-like Receptor3-mediated Induction of Chemokines in Salivary Epithelial Cells
The Korean Journal of Physiology and Pharmacology
;
: 235-240, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-727794
ABSTRACT
Toll-like receptors (TLRs) functionally expressed in salivary epithelial cells, but their roles remain elusive. Among TLRs family, TLR3 is activated by dsRNA, a byproduct of viral infection. The aim of this study was to investigate the role of TLR3 in the inflammatory immune responses using HSG cells. Reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR and ELISA were performed to identify expression of TLRs and TLR3-mediated chemokine inductions. The chemotaxis assay of activated T lymphocytes was also performed. Treatment of HSG cells with polyinosinic polycytidylic acid (poly(IC)) significantly increased interferon-gamma-inducible protein 10 (IP-10), interferoninducible T-cell alpha chemoattractant (I-TAC), and regulated on activation, normal T-cells expressed and secreted (RANTES) gene expressions in a concentration-dependent manner. Anti-TLR3 antibody blocked the increases of IP-10 and I-TAC genes. Poly(IC)-induced increases of IP-10 and I-TAC were also confirmed at protein levels from cell lysates, but their release into extracellular medium was detected only in IP-10. We found that the culture media from HSG cells stimulated with poly(IC) significantly increases T lymphocyte migration. Our results suggest that TLR3 plays an important role in chemokine induction, particularly IP-10, in salivary epithelial cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Linfocitos
/
Linfocitos T
/
Expresión Génica
/
Quimiotaxis
/
Quimiocinas
/
Medios de Cultivo
/
Células Epiteliales
/
Receptores Toll-Like
/
Reacción en Cadena en Tiempo Real de la Polimerasa
Límite:
Humanos
Idioma:
Inglés
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2010
Tipo del documento:
Artículo
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