Vibrio vulnificus Cytolysin Forms Anion-selective Pores on the CPAE Cells, a Pulmonary Endothelial Cell Line
The Korean Journal of Physiology and Pharmacology
;
: 259-264, 2004.
Artículo
en Inglés
| WPRIM
| ID: wpr-727915
ABSTRACT
Cytolysin produced by Vibrio vulnificus has been incriminated as one of the important virulence determinants in V. vulnificus infection. Ion selectivity of cytolysin-induced pores was examined in a CPAE cell, a cell line of pulmonary endothelial cell, using inside-out patch clamp techniques. In symmetrical NaCl concentration (140 mM), intracellular or extracellular application of cytolysin formed ion-permeable pores with a single channel conductance of 37.5 4.0 pS. The pore currents were consistently maintained after washout of cytolysin. Replacement of Na in bath solution with monovalent ions (K, Cs or TEA ) or with divalent ions (Mg2, Ca2 ) did not affect the pore currents. When the NaCl concentration in bath solution was lowered from 140 to 60 and 20 mM, the reversal potential shifted from 0 to 11.8 and 28.2 mV, respectively. The relative permeability of the cytolysin pores to anions measured at 40 mV was Cl = NO2 > or = Br = I > SCN > acetate > isethionate > ascorbic acid > EDTA2, in descending order. The cytolysin-induced pore current was blocked by Cl channel blockers or nucleotides. These results indicate that V. vulnificus cytolysin forms anion-selective pores in CPAE cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Permeabilidad
/
Ácido Ascórbico
/
Té
/
Vibrio
/
Baños
/
Virulencia
/
Línea Celular
/
Técnicas de Placa-Clamp
/
Vibrio vulnificus
/
Células Endoteliales
Idioma:
Inglés
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2004
Tipo del documento:
Artículo
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