Detection of Mitochondrial ATP-Sensitive Potassium Channels in Rat Cardiomyocytes
The Korean Journal of Physiology and Pharmacology
;
: 201-206, 2004.
Artículo
en Inglés
| WPRIM
| ID: wpr-727923
ABSTRACT
Mitochondrial ATP-sensitive potassium (mitoKATP) channels play a role in early and late ischemic preconditioning. Nevertheless, the subunit composition of mitoKATP channels remains unclear. In this study, we investigated the subunit composition of mitoKATP channels in mitochondria isolated from rat cardiac myocytes. Mitochondria were visualized using the red fluorescence probe, Mitrotracker Red, while mitoKATP channels were visualized using the green fluorescence probe, glibenclamide-BODIPY. The immunofluorescence confocal microscopy revealed the presence of Kir6.1, Kir6.2 and SUR2 present in the cardiac mitochondria. Western blot analysis was carried to further investigate the nature of mitoKATP channels. For SUR proteins, a 140-kDa immunoreactive band that corresponded to SUR2, but no SUR1 was detected. For Kir6.2, three bands (~4, ~6, and ~0 kDa) were detected, and a specific ~6-kDa immunoreactive band corresponding to Kir6.1 was also observed. These observations suggest that the subunits of mitoKATP channels in rat myocytes include Kir6.1, Kir6.2, and a SUR2-related sulfonylurea-binding protein.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Potasio
/
Western Blotting
/
Técnica del Anticuerpo Fluorescente
/
Microscopía Confocal
/
Precondicionamiento Isquémico
/
Células Musculares
/
Miocitos Cardíacos
/
Canales KATP
/
Fluorescencia
/
Mitocondrias
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2004
Tipo del documento:
Artículo
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