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Effects of Cl- channel blockers on the cardiac ATP-sensitive K+ channel
The Korean Journal of Physiology and Pharmacology ; : 305-313, 1999.
Artículo en Inglés | WPRIM | ID: wpr-728244
ABSTRACT
To explore whether Cl- channel blockers interact with the ATP-sensitive K+ (KATP) channel, I have examined the effect of two common Cl- channel blockers on the KATP channel activity in isolated rat ventricular myocytes using patch clamp techniques. In inside-out patches, 4,4'-diisothio-cyanatostilbene-2,2'-disulfonic acid (DIDS) and niflumic acid applied to bath solution inhibited the KATP channel activity in a concentration-dependent manner with IC50 of 0.24 and 927 muM, respectively. The inhibitory action of DIDS was irreversible whereas that of niflumic acid was reversible. Furthermore, DIDS-induced block was not recovered despite exposure to ATP (1 mM). In cell-attached and inside-out patches, DIDS blocked the pinacidil- or 2,4-dinitrophenol (DNP)-induced KATP channel openings. In contrast, niflumic acid did not block the pinacidil-induced KATP channel openings in inside-out patches, but inhibited it in cell-attached patches. DIDS and niflumic acid produced additional block in the presence of ATP and did not affect current-voltage relationship and channel kinetics. All these results indicate that DIDS among Cl- channel blockers specifically blocks the cardiac KATP channel.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Baños / Cinética / Adenosina Trifosfato / Ácido Niflúmico / Ácido 4,4'-Diisotiocianostilbeno-2,2'-Disulfónico / Técnicas de Placa-Clamp / 2,4-Dinitrofenol / Concentración 50 Inhibidora / Células Musculares Tipo de estudio: Estudio diagnóstico Límite: Animales Idioma: Inglés Revista: The Korean Journal of Physiology and Pharmacology Año: 1999 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Baños / Cinética / Adenosina Trifosfato / Ácido Niflúmico / Ácido 4,4'-Diisotiocianostilbeno-2,2'-Disulfónico / Técnicas de Placa-Clamp / 2,4-Dinitrofenol / Concentración 50 Inhibidora / Células Musculares Tipo de estudio: Estudio diagnóstico Límite: Animales Idioma: Inglés Revista: The Korean Journal of Physiology and Pharmacology Año: 1999 Tipo del documento: Artículo