Multiple Regulation of Roundabout (Robo) Phosphorylation in a Heterologous Cell System
The Korean Journal of Physiology and Pharmacology
;
: 111-115, 2004.
Artículo
en Inglés
| WPRIM
| ID: wpr-728494
ABSTRACT
Roundabout (Robo) is the transmembrane receptor for slit, the neuronal guidance molecule. In this study, the tyrosine phosphorylation of Robo was observed in Robo-transfected human embryonic kidney cells and developing rat brains, and found to be increased by the treatment with protein kinase A activator, forskolin. In contrast, protein kinase C activation by phorbol-12-myristate-13-acetate decreased the phosphorylation of Robo. Intracellular calcium was required for the tyrosine phosphorylation. Furthermore, the transfection of an Eph receptor tyrosine kinase dramatically enhanced the tyrosine phosphorylation. These findings indicate that the tyrosine phosphorylation of Robo is regulated by multiple mechanisms, and that Eph receptor kinases may play a role in the regulation of tyrosine phosphorylation of Robo in the rat brain.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosforilación
/
Fosfotransferasas
/
Proteínas Quinasas
/
Tirosina
/
Encéfalo
/
Colforsina
/
Proteína Quinasa C
/
Transfección
/
Calcio
/
Proteínas Quinasas Dependientes de AMP Cíclico
Tipo de estudio:
Guía de Práctica Clínica
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2004
Tipo del documento:
Artículo
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